Recombinant trehalose-6-phosphate phosphatase of Brugia malayi cross-reacts with human Wuchereria bancrofti immune sera and engenders a robust protective outcome in mice.
Identifieur interne : 003F33 ( Main/Exploration ); précédent : 003F32; suivant : 003F34Recombinant trehalose-6-phosphate phosphatase of Brugia malayi cross-reacts with human Wuchereria bancrofti immune sera and engenders a robust protective outcome in mice.
Auteurs : Susheela Kushwaha [Inde] ; Prashant Kumar Singh ; Jyoti Gupta ; Vishal Kumar Soni ; Shailja Misra-BhattacharyaSource :
- Microbes and infection [ 1769-714X ] ; 2012.
Descripteurs français
- KwdFr :
- Analyse de variance, Animaux, Anticorps antihelminthe (immunologie), Anticorps antihelminthe (métabolisme), Anticorps antihelminthe (sang), Biologie informatique, Brugia malayi (enzymologie), Brugia malayi (génétique), Brugia malayi (immunologie), Cytokines (immunologie), Cytokines (métabolisme), Données de séquences moléculaires, Filariose lymphatique (immunologie), Immunoglobuline G (immunologie), Immunoglobuline G (métabolisme), Immunoglobuline G (sang), Macrophages, Mâle, Phosphoric monoester hydrolases (immunologie), Phosphoric monoester hydrolases (métabolisme), Prolifération cellulaire, Protéines d'helminthes (immunologie), Protéines d'helminthes (métabolisme), Protéines recombinantes (immunologie), Protéines recombinantes (métabolisme), Réactions croisées, Simulation numérique, Souris, Souris de lignée BALB C, Séquence d'acides aminés, Vaccins synthétiques (immunologie), Wuchereria bancrofti (génétique), Wuchereria bancrofti (immunologie).
- MESH :
- enzymologie : Brugia malayi.
- génétique : Brugia malayi, Wuchereria bancrofti.
- immunologie : Anticorps antihelminthe, Brugia malayi, Cytokines, Filariose lymphatique, Immunoglobuline G, Phosphoric monoester hydrolases, Protéines d'helminthes, Protéines recombinantes, Vaccins synthétiques, Wuchereria bancrofti.
- métabolisme : Anticorps antihelminthe, Cytokines, Immunoglobuline G, Phosphoric monoester hydrolases, Protéines d'helminthes, Protéines recombinantes.
- sang : Anticorps antihelminthe, Immunoglobuline G.
- Analyse de variance, Animaux, Biologie informatique, Données de séquences moléculaires, Macrophages, Mâle, Prolifération cellulaire, Réactions croisées, Simulation numérique, Souris, Souris de lignée BALB C, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Analysis of Variance, Animals, Antibodies, Helminth (blood), Antibodies, Helminth (immunology), Antibodies, Helminth (metabolism), Brugia malayi (enzymology), Brugia malayi (genetics), Brugia malayi (immunology), Cell Proliferation, Computational Biology, Computer Simulation, Cross Reactions, Cytokines (immunology), Cytokines (metabolism), Elephantiasis, Filarial (immunology), Helminth Proteins (immunology), Helminth Proteins (metabolism), Immunoglobulin G (blood), Immunoglobulin G (immunology), Immunoglobulin G (metabolism), Macrophages, Male, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Phosphoric Monoester Hydrolases (immunology), Phosphoric Monoester Hydrolases (metabolism), Recombinant Proteins (immunology), Recombinant Proteins (metabolism), Vaccines, Synthetic (immunology), Wuchereria bancrofti (genetics), Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth, Immunoglobulin G.
- chemical , immunology : Antibodies, Helminth, Cytokines, Helminth Proteins, Immunoglobulin G, Phosphoric Monoester Hydrolases, Recombinant Proteins, Vaccines, Synthetic.
- chemical , metabolism : Antibodies, Helminth, Cytokines, Helminth Proteins, Immunoglobulin G, Phosphoric Monoester Hydrolases, Recombinant Proteins.
- enzymology : Brugia malayi.
- genetics : Brugia malayi, Wuchereria bancrofti.
- immunology : Brugia malayi, Elephantiasis, Filarial, Wuchereria bancrofti.
- Amino Acid Sequence, Analysis of Variance, Animals, Cell Proliferation, Computational Biology, Computer Simulation, Cross Reactions, Macrophages, Male, Mice, Mice, Inbred BALB C, Molecular Sequence Data.
Abstract
Trehalose-6-phosphate phosphatase of Brugia malayi (Bm-TPP) represents an attractive vaccine candidate because it is present in all the major life stages of parasite, but is absent in mammals. We have previously cloned, purified and biochemically characterized Bm-TPP. In the present study, we investigated the cross-reactivity of recombinant Bm-TPP (r-Bm-TPP) with the sera of human bancroftian patients belonging to different disease categories. In silico study using bioinformatics tool demonstrated that Bm-TPP is highly immunogenic in nature. BALB/c mice administered with r-Bm-TPP alone or in combination with Freund's complete adjuvant (FCA) generated a strong IgG response. Further investigations on the antibody isotypes showed generation of a mixed T helper cell response which was marginally biased towards Th1 phenotype. r-Bm-TPP with or without adjuvant lead to significantly increased accumulation of CD4+ and CD8+ T cells in the spleen of infected mice and increased the activation of peritoneal macrophages. Additionally, r-Bm-TPP enhanced the production of both proinflammatory (IL-2, IFN-γ) and anti-inflammatory (IL-4, IL-10) cytokines and mice immunized with r-Bm-TPP alone or in combination with FCA showed 54.5% and 67% protection respectively against B. malayi infective larvae challenge. Taken together, our findings suggest that Bm-TPP is protective in nature and might be a potential candidate for development of vaccine against lymphatic filarial infections.
DOI: 10.1016/j.micinf.2012.08.006
PubMed: 22981601
Affiliations:
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(blood)</term><term>Immunoglobulin G (immunology)</term><term>Immunoglobulin G (metabolism)</term><term>Macrophages</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Phosphoric Monoester Hydrolases (immunology)</term><term>Phosphoric Monoester Hydrolases (metabolism)</term><term>Recombinant Proteins (immunology)</term><term>Recombinant Proteins (metabolism)</term><term>Vaccines, Synthetic (immunology)</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de variance</term><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Anticorps antihelminthe (métabolisme)</term><term>Anticorps antihelminthe (sang)</term><term>Biologie informatique</term><term>Brugia malayi (enzymologie)</term><term>Brugia malayi (génétique)</term><term>Brugia malayi (immunologie)</term><term>Cytokines 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aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Trehalose-6-phosphate phosphatase of Brugia malayi (Bm-TPP) represents an attractive vaccine candidate because it is present in all the major life stages of parasite, but is absent in mammals. We have previously cloned, purified and biochemically characterized Bm-TPP. In the present study, we investigated the cross-reactivity of recombinant Bm-TPP (r-Bm-TPP) with the sera of human bancroftian patients belonging to different disease categories. In silico study using bioinformatics tool demonstrated that Bm-TPP is highly immunogenic in nature. BALB/c mice administered with r-Bm-TPP alone or in combination with Freund's complete adjuvant (FCA) generated a strong IgG response. Further investigations on the antibody isotypes showed generation of a mixed T helper cell response which was marginally biased towards Th1 phenotype. r-Bm-TPP with or without adjuvant lead to significantly increased accumulation of CD4+ and CD8+ T cells in the spleen of infected mice and increased the activation of peritoneal macrophages. Additionally, r-Bm-TPP enhanced the production of both proinflammatory (IL-2, IFN-γ) and anti-inflammatory (IL-4, IL-10) cytokines and mice immunized with r-Bm-TPP alone or in combination with FCA showed 54.5% and 67% protection respectively against B. malayi infective larvae challenge. Taken together, our findings suggest that Bm-TPP is protective in nature and might be a potential candidate for development of vaccine against lymphatic filarial infections.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Gupta, Jyoti" sort="Gupta, Jyoti" uniqKey="Gupta J" first="Jyoti" last="Gupta">Jyoti Gupta</name><name sortKey="Misra Bhattacharya, Shailja" sort="Misra Bhattacharya, Shailja" uniqKey="Misra Bhattacharya S" first="Shailja" last="Misra-Bhattacharya">Shailja Misra-Bhattacharya</name><name sortKey="Singh, Prashant Kumar" sort="Singh, Prashant Kumar" uniqKey="Singh P" first="Prashant Kumar" last="Singh">Prashant Kumar Singh</name><name sortKey="Soni, Vishal Kumar" sort="Soni, Vishal Kumar" uniqKey="Soni V" first="Vishal Kumar" last="Soni">Vishal Kumar Soni</name></noCountry><country name="Inde"><noRegion><name sortKey="Kushwaha, Susheela" sort="Kushwaha, Susheela" uniqKey="Kushwaha S" first="Susheela" last="Kushwaha">Susheela Kushwaha</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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